Thermo Fisher Scientific CD73 Polyclonal Antibody
다양한 종에서 반응하는 Rabbit Polyclonal CD73 항체로, WB, IHC, ICC, Flow, ELISA 등 다양한 응용에 사용 가능. 단백질 A 정제, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| Flow Cytometry (Flow) | 1 µg/test |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Bovine, Dog, Chicken, Human, Mouse, Sheep, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human CD73/NT5, amino acids 151–250 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD73 (Ecto-5'-nucleotidase, 5'-ribonucleotide phosphohydrolase) is an enzyme that catalyzes the conversion of purine 5'-mononucleotides to nucleosides at neutral pH, with AMP as the preferred substrate. It forms a dimer of two identical 70 kDa subunits bound to the plasma membrane via a glycosyl phosphatidylinositol linkage. CD73 serves as a marker for lymphocyte differentiation, and its deficiency is associated with various immunodeficiency diseases. Other 5'-nucleotidase forms exist in the cytoplasm and lysosomes, distinguishable by substrate affinity and cofactor requirements. The CD73 gene is localized to chromosome 6q14–q21. Defects in this gene can cause joint and arterial calcification as well as intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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