Thermo Fisher Scientific PLK1 Monoclonal Antibody (13E8)
PLK1 단백질을 검출하기 위한 마우스 단클론 항체로, 인간·생쥐·랫트 시료에 반응. Western blot, IHC, ICC, IP 등 다양한 응용 가능. Protein G 정제, 1 mg/mL 농도, PBS+BSA buffer에 보존. 세포분열 및 암 연구용으로 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:20 | - |
| Immunocytochemistry (ICC/IF) | 3 µg/mL | - |
| ELISA | - | View 1 publication |
| Immunoprecipitation (IP) | Assay-dependent | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Human |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13E8 |
| Immunogen | Recombinant protein containing residues 300–400 of human PLK1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS with 1 mg/mL BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2237361 |
Product Specific Information
MA1-848 detects PLK1 from human, mouse, and rat samples.
It has been successfully used in Western blot, immunoprecipitation, and immunofluorescence procedures.
The immunogen is a recombinant protein containing residues 300–400 of human PLK1.
Target Information
PLK1 is a serine/threonine kinase involved in cell mitosis and is regarded as a marker of cell proliferation.
It plays a critical role in carcinogenesis. Polo-like kinase proteins bind and phosphorylate proteins already phosphorylated on a specific motif recognized by the POLO box domains.
PLK1 phosphorylates multiple targets including BORA, BUB1B/BUBR1, CCNB1, CDC25C, CEP55, ECT2, ERCC6L, FBXO5/EMI1, FOXM1, KIF20A/MKLP2, CENPU, NEDD1, NINL, NPM1, NUDC, PKMYT1/MYT1, KIZ, PPP1R12A/MYPT1, PRC1, RACGAP1/CYK4, SGOL1, STAG2/SA2, TEX14, TOPORS, TP73, TPT1, and WEE1.
It is essential for centrosome functions and bipolar spindle assembly by phosphorylating KIZ, NEDD1, and NINL.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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