Thermo Fisher Scientific Phospho-TH (Ser31) Polyclonal Antibody
도파민 및 카테콜아민 합성 효소인 Tyrosine Hydroxylase의 Ser31 인산화 형태를 특이적으로 인식하는 폴리클로날 항체입니다. WB, IHC, ICC, ELISA 등 다양한 응용에 적합하며, 토끼 유래 IgG 형식으로 높은 특이성과 재현성을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic phosphopeptide derived from rat TH around the phosphorylation site of Ser31 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Tyrosine hydroxylase (TH) is an enzyme involved in the synthesis of catecholamine neurotransmitters including dopamine, epinephrine, and norepinephrine. Catecholamine synthesis is regulated by the interaction of TH with the cofactor tetrahydrobiopterin (BH4), which binds to the TH catalytic domain to activate the enzyme.
In humans, four TH mRNA splice variants (hTH1–hTH4) have been identified, sharing the same catalytic domain but differing in their N-terminal regulatory regions. TH catalyzes the conversion of L-tyrosine to L-DOPA and plays a key role in adrenergic neuron function. Dysregulation of TH is associated with neurological and cardiovascular disorders such as Parkinson’s disease, schizophrenia, Segawa syndrome, and dystonia.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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