Thermo Fisher Scientific Kappa Light Chain (B-Cell Marker) Monoclonal Antibody (SPM558)
인간 Kappa Light Chain을 표적으로 하는 Mouse monoclonal antibody (Clone SPM558). IHC 및 Flow Cytometry에 적합하며, Protein A/G로 정제된 200 µg/mL 액상 형태. PBS buffer(0.05% BSA, sodium azide 포함)로 보관하며 4°C에서 안정적.
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Thermo Fisher Scientific Kappa Light Chain (B-Cell Marker) Monoclonal Antibody (SPM558)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | Assay-dependent |
| Immunohistochemistry (PFA fixed) (IHC (PFA)) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | SPM558 |
| Immunogen | Recombinant human Ig kappa chain |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS, pH 7.4, with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Immunoglobulin classes share the same basic four polypeptide chain structure consisting of two heavy chains (five types) and two light chains (kappa and lambda, both with a molecular weight of 22.5 kDa).
Kappa and lambda chains each have a variable and a constant region, which can be differentiated by antigenic properties of the constant region. The typical ratio of kappa to lambda is approximately 70:30.
Antibodies to the kappa light chain are useful in identifying leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. Demonstration of clonality in lymphoid infiltrates indicates malignancy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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