Thermo Fisher Scientific Phospho-STAT5 alpha (Ser725) Polyclonal Antibody
Rabbit polyclonal antibody recognizing phospho-STAT5 alpha (Ser725). Validated for WB, IHC, and ICC/IF applications. Supplied as 1 mg/mL liquid, unconjugated form. Suitable for research on cytokine and growth factor signaling pathways.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| Property | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human STAT5A around the phosphorylation site of Ser725 (A-P-SP-P-V) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2555126 |
Target Information
STAT5 alpha is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by receptor-associated kinases, forming homo- or heterodimers that translocate to the nucleus and act as transcription activators. STAT5 alpha mediates responses to ligands such as IL2, IL3, IL7, GM-CSF, erythropoietin, thrombopoietin, and growth hormones. Activation of STAT5 alpha in myeloma and lymphoma associated with TEL/JAK2 gene fusion is independent of cell stimulus and essential for tumorigenesis.
In mice, STAT5 alpha induces expression of BCL2L1/BCL-X(L), suggesting an antiapoptotic role. STAT5 alpha and STAT5 beta share 96% sequence similarity and are co-expressed in most tissues, with differential mRNA accumulation in muscle and mammary tissue. STAT5 alpha is critically involved in reproduction, lactation, immune function, and somatic growth.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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