Thermo Fisher Scientific CD25 Monoclonal Antibody (PC61.5), Super Bright 702, eBioscience
마우스 CD25(IL-2Rα)를 인식하는 PC61.5 단클론 항체로, Super Bright 702 형광체가 결합된 제품입니다. Flow cytometry에 최적화되어 있으며, 405 nm 레이저로 여기되어 702 nm에서 방출됩니다. 4°C 암소 보관, 연구용 전용입니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Immunohistochemistry (IHC) | - | 1 publication |
| Flow Cytometry (Flow) | 0.125 µg/test | 46 publications |
| Neutralization (Neu) | - | 1 publication |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Rat / IgG1, lambda |
| Recommended Isotype Control | Rat IgG1 kappa Isotype Control (eBRG1), Super Bright™ 702, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PC61.5 |
| Conjugate | Super Bright™ 702 |
| Excitation / Emission Max | 413 / 702 nm |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C, store in dark, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2717141 |
Additional Available Formats
Unconjugated, Alexa Fluor 488, 532, 561, 647, 660, 700, APC, APC-eFluor 780, Biotin, Brilliant UV 563/661/737/805, Brilliant Violet 421/650/711/786, eFluor 450/506, FITC, Functional Grade, NovaFluor Blue series, Pacific Orange, PE, PE-Cyanine5/5.5/7, PE-eFluor 610, PerCP-Cyanine5.5, PerCP-eFluor 710, Super Bright 436/600/645/780, custom conjugation available.
Product Specific Information
The PC61.5 antibody reacts with mouse CD25, the 55 kDa interleukin-2 receptor alpha chain (IL-2Rα). CD25 is expressed by early T and B cell progenitors and activated mature lymphocytes. It binds IL-2 with low affinity alone but forms a high-affinity receptor complex with CD122 (IL-2Rβ) and CD132 (common γ chain). The PC61.5 antibody inhibits IL-2 binding to both high and low affinity receptors and plays a role in lymphocyte activation and proliferation.
Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes at ≤0.125 µg/test (100 µL final volume). Optimize concentration empirically.
Super Bright 702 Properties:
- Tandem dye excited by 405 nm (violet laser), emission at 702 nm
- Recommended filter: 710/50 bandpass
- Use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize non-specific interactions
- Light sensitive: protect from light
- Fixation: compatible with IC Fixation Buffer (Product # 00-8222) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days at 4°C in dark
- Excitation: 405 nm / Emission: 702 nm / Laser: Violet
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
CD25 (IL2RA) encodes the alpha chain of the interleukin-2 receptor, involved in T and B lymphocyte proliferation. The receptor complex (IL2RA, IL2RB, IL2RG) forms a high-affinity IL-2 receptor. Homodimeric IL2RA or IL2RB forms lower-affinity receptors. IL-2 is produced by mature T cells and regulates growth, differentiation, and apoptosis. Mutations in IL2RA are linked to immune dysregulation and colitis-like disease in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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