Thermo Fisher Scientific PC4 Polyclonal Antibody
Rabbit polyclonal antibody against human PC4, suitable for WB, IHC, and IP applications. Recognizes residues 77–127 of human PC4. Recommended for research use only, with 1-year shelf life and storage at 4°C.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500–1:2,000 |
| Immunoprecipitation (IP) | Assay-dependent |
Product Specifications
| Specification | Detail |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 77 and 127 of human positive cofactor 4 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
- Based on sequence identity, this antibody is predicted to react with Rat, Chicken, and Orangutan.
Target Information
PC4 (activated RNA polymerase II transcription cofactor 4) is a transcriptional coactivator that can suppress both promoter-driven and nonspecific transcription through its DNA-binding activity.
The repressive activity of PC4 on promoter-driven transcription is alleviated by transcription factor TFIIH, which protects promoters from PC4-mediated repression by relieving the topological constraint imposed by PC4 via ERCC3 helicase activity.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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