Thermo Fisher Scientific DGKQ Polyclonal Antibody
Rabbit polyclonal antibody against human DGKQ for Western blot applications. Recognizes multiple species with high homology. Supplied as a liquid form at 0.5 mg/mL, purified by affinity chromatography. Store at -20°C; avoid freeze-thaw cycles.
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Applications
- Western Blot (WB): Tested dilution 0.2–1 µg/mL
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide directed towards the middle region of human DGKQ |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 2% sucrose |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2884472 |
Product Specific Information
Immunogen sequence:
DAELSLDFHQ AREEEPGKFT SRLHNKGVYV RVGLQKISHS RSLHKQIRLQ
For short-term use, store at 2–8°C up to 1 week. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Predicted Homology:
Cow: 100%
Dog: 86%
Guinea Pig: 93%
Horse: 100%
Human: 100%
Mouse: 100%
Pig: 100%
Rat: 100%
Target Information
Diacylglycerol (DAG) is a fundamental lipid second messenger produced in the nucleus. The accumulation of DAG in the nucleus regulates cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, terminating DAG signaling, reducing protein kinase C activity, and controlling T lymphocyte cell cycle progression.
DGKs are divided into five subtypes (Type I–V). DGK-theta is a Type V DGK localized mainly in the nucleus of various cell lines (MDA-MB-453, MCF-7, PC12, HeLa). Nuclear DGK-theta co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP₂) and responds to alpha-Thrombin stimulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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