Thermo Fisher Scientific Glu-Glu Tag Polyclonal Antibody
Glu-Glu 태그 단백질 검출용 염소 폴리클로날 항체로, WB, ICC/IF, ELISA, IP 등 다양한 응용에 적합합니다. EYMPME 서열 기반 합성 펩타이드로 면역화되었으며, 항원 친화 크로마토그래피로 정제되었습니다. 비접합 액상 형태로 4°C에서 보관합니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 - 1:30,000 |
| Immunocytochemistry (ICC/IF) | 1:100 - 1:400 |
| ELISA | 1:1,000 - 1:30,000 |
| Immunoprecipitation (IP) | 1 - 4 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Tag |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide representing amino acid residues 314–319 (EYMPME) of middle T antigen of mouse polyomavirus conjugated to KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 6.8–7.4 |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Goats were immunized with a synthetic peptide representing amino acid residues 314–319 (EYMPME) of the middle T antigen of mouse polyomavirus conjugated to KLH. The antibody was isolated by affinity chromatography using the peptide immobilized on a solid support.
Target Information
The Glu-Glu tag (EYMPME sequence) is a short peptide epitope tag containing two glutamic acid (Glu) residues interspersed with other amino acids. It is typically incorporated into the N-terminus of proteins via subcloning into vectors such as the pFASTBAC vector. This tag is widely used in recombinant protein expression and purification, enabling detection and isolation of tagged proteins through specific antibodies recognizing the Glu-Glu sequence. It is applicable in various expression systems, including baculovirus-infected insect cells, and facilitates studies on protein-protein interactions, localization, and function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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