Thermo Fisher Scientific MEK2 Polyclonal Antibody
MEK2 단백질을 인식하는 Rabbit Polyclonal 항체로 Western blot, IHC, ICC/IF에 사용 가능. Human에 반응하며 Mouse, Rat 등과 교차 반응 예측. 항원 친화 크로마토그래피로 정제된 액상 제품으로 안정적 보관 가능.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:5,000–1:25,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fragment corresponding to a region within amino acids 188–396 of Human MEK2 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.97 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7, with 1% BSA, 20% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2545111 |
Product Specific Information
- Recommended positive controls: 293T, A431, Jurkat, Raji
- Predicted reactivity: Mouse (91%), Rat (92%), Dog (93%), Pig (93%), Chicken (91%), Bovine (93%)
- Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
MAP2K2 (MEK2) is a dual specificity serine/threonine kinase involved in mitogen growth factor signal transduction.
MEK2 (MAPKK2) activates ERK1/2 and MAPK enzymes by phosphorylating residues within the TEY motif.
MEK1 and MEK2 are activated by dual phosphorylation on serine 218 and 222.
Threonine 292 of MEK1 is phosphorylated by ERK2, providing a negative feedback loop that suppresses MEK1 activation.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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