Thermo Fisher Scientific Phospho-MAP2K1/MAP2K2 (Ser218, Ser222, Ser226) Polyclonal Antibody
MEK1/2의 Ser218, Ser222, Ser226 인산화 상태를 특이적으로 검출하는 폴리클로날 항체. Western blot, IHC, ICC/IF에 적합. 인간, 마우스, 랫트 반응성. 고순도 정제 및 안정적 액상 형태로 연구용에 최적화.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human MAP2K1 (Accession Q02750), corresponding to amino acid residues around phosphorylated Ser226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817176 |
Product Specific Information
Antibody detects endogenous levels of MEK1/2 only when phosphorylated at S218+S222 (MEK1) / S222+S226 (MEK2).
Target Information
MEK1 (MAP Kinase Kinase, also known as MKK) is an integral component of the MAP kinase cascade that regulates cell growth and differentiation (Ahn, 1993; Chong et al., 2003). In brain, this pathway also plays a key role in synaptic signaling (Adams and Sweatt, 2002). Activated MEK1 acts as a dual specificity kinase phosphorylating both threonine and tyrosine residues on MAP kinase (Kyriakis et al., 1991; Seger et al., 1991; Crews et al., 1992).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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