Thermo Fisher Scientific PTPN12 Polyclonal Antibody
Rabbit polyclonal antibody recognizing human PTPN12 (PTP PEST). Validated for WB, IHC, IHC-P, and IP applications. Supplied as liquid, unconjugated form at 0.2 mg/mL concentration. Recommended storage at 4°C with 1-year shelf life.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (IHC) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:100–1:500 |
| Immunoprecipitation (IP) | 2–5 µg/mg lysate |
Product Specifications
| Specification | Description |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 760 and 780 of human protein tyrosine phosphatase, non-receptor type 12 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- Application Note: For IHC, epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections
Target Information
PTP PEST is an 88 kDa cytosolic protein tyrosine phosphatase ubiquitously expressed in mammalian tissues. It exhibits high specific activity with artificial tyrosine-phosphorylated substrates in vitro. PTP PEST is regulated via phosphorylation of serine 39, catalyzed by protein kinase C and protein kinase A.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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