Thermo Fisher Scientific CaMKIV Recombinant Rabbit Monoclonal Antibody (1G4P4)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:500-1:1,000

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

1G4P4

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 374-473 of human CAMKIV (Q16566). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:CaMKIV,uniProtId:Q16566-1,ncbiNodeId:9606,antigenRange:374-473,antigenLength:473,antigenImageFileName:MA5-42919_CaMKIV_Q16566-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42919_CaMKIV_Q16566-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.18 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2912060

Product Specific Information

Positive test controls include: SH-SY5Y, Mouse thymus.

Immunogen sequence: EGEKIQGDGA QAAVKGAQAE LMKVQALEKV KGADINAEEA PKMVPKAVED GIKVADLELE EGLAEEKLKT VEEAAAPREG QGSSAVGFEV PQQDVILPEY

Target Information

CAMK4 (CaMKIV) is a serine/threonine protein kinase that phosphoryates CREB, oncoprotein 18, and SRF. It has limited tissue distribution, and has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells. The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.