Thermo Fisher Scientific Phospho-ZIP Kinase (Thr265) Polyclonal Antibody
Phospho-ZIP Kinase (Thr265) 단백질의 인산화 상태를 검출하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC, ELISA 등에 적합하며, Human, Mouse, Rat 시료에 반응. Protein A로 정제된 고순도 액상 항체로 연구용에 적합.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry / Immunofluorescence (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Detail |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthesized phosphopeptide derived from human ZIP Kinase around the phosphorylation site of Thr265 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
DAPK3 (Death-associated protein kinase 3) is a serine/threonine kinase involved in cell motility, smooth muscle contractility, and cell death processes. It is encoded by the ZIPK gene and functions as a component of desmosomes and the epidermal cornified envelope in keratinocytes.
The N-terminal domain interacts with the plasma membrane, while the C-terminus associates with intermediate filaments. Through its rod domain, DAPK3 forms complexes with envoplakin, linking the cornified envelope, desmosomes, and intermediate filaments.
AKT1/PKB, a kinase involved in cell growth and survival signaling, interacts with DAPK3, suggesting its role as a localization signal in AKT1-mediated pathways.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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