Thermo Fisher Scientific ERK5 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 1 publication 1 publication

Immunoprecipitation (IP)

1:50

-

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to human Erk5

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

23 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

no preservative

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10981943

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with Erk1 or Erk2.

Target Information

MAPK7 (ERK5) serine/threonine kinase is a member of the MAP kinase family and is involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. MAPK7 is activated by mitogen-activated protein kinase 5 (MAP2K5/MEK5) and is involved in downstream signaling processes of various receptors. In response to extracelluar signals, MAPK7 translocates to the cell nucleus where it regulates gene expression by phosphorylating and activating different transcription factors. Gene deletion of MAPK7 in mice results in defective blood vessel and cardiac development leading to embryonic lethality. MAPK7 has been shown to be critical for endothelial function and maintenance of blood vessel integrity. Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported. ERK5 is expressed in many adult tissues, abundantly in heart, placenta, lung, kidney and skeletal muscle, but is not detectable in liver.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.