Thermo Fisher Scientific GCK Polyclonal Antibody
Thermo Fisher Scientific의 GCK Polyclonal Antibody는 인간, 마우스, 랫트 시료에 반응하며 Western blot 및 ELISA에 적합합니다. Rabbit IgG 폴리클로날 항체로, 정제된 액상 형태이며 PBS/glycerol 버퍼에 보관됩니다. Glucokinase 연구용으로 설계되었습니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| ELISA | 1 µg/mL | - |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–300 of human Glucokinase (GCK) (NP_0001531) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 4.21 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | −20 °C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806525 |
Product Specific Information
Immunogen sequence:
MLDDRARMEA AKKEKVEQIL AEFQLQEEDL KKVMRRMQKE MDRGLRLETH EEASVKMLPT YVRSTPEGSE VGDFLSLDLG GTNFRVMLVK VGEGEEGQWS VKTKHQMYSI PEDAMTGTAE MLFDYISECI SDFLDKHQMK HKKLPLGFTF SFPVRHEDID KGILLNWTKG FKASGAEGNN VVGLLRDAIK RRGDFEMDVV AMVNDTVATM ISCYYEDHQC EVGMIVGTGC NACYMEEMQN VELVEGDEGR MCVNTEWGAF GDSGELDEFL LEYDRLVDES SANPGQQLYE KLIGGKYMGE
Positive Samples: Mouse liver, Rat skeletal muscle
Cellular Location: Cytoplasm, Nucleus
Target Information
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, committing glucose to the glycolytic pathway. Alternative splicing of this gene results in three tissue-specific forms of glucokinase: one in pancreatic islet beta cells and two in liver. The protein localizes to the outer membrane of mitochondria. Unlike other hexokinases, this enzyme is not inhibited by glucose-6-phosphate and remains active when glucose is abundant. Mutations in this gene are associated with non-insulin dependent diabetes mellitus (NIDDM), maturity onset diabetes of the young type 2 (MODY2), and persistent hyperinsulinemic hypoglycemia of infancy (PHHI).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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