Thermo Fisher Scientific SHC2/Sck Polyclonal Antibody
SHC2/Sck 단백질을 인식하는 토끼 폴리클로날 항체로, IHC, ICC/IF, ELISA 등 다양한 응용에 적합합니다. 인간, 마우스, 랫트 반응성 보유하며 Protein A로 정제된 고순도 제품입니다. 신호전달 연구 및 VEGF 관련 경로 분석에 유용합니다.
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Thermo Fisher Scientific SHC2/Sck Polyclonal Antibody
Applications 및 희석 배수
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human SHC2/Sck (amino acids 161–270) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage buffer | 0.01M TBS, pH 7.4, with 1% BSA, 50% glycerol |
| Contains | 0.02% ProClin 300 |
| Storage conditions | -20°C |
| Shipping conditions | Ambient (domestic); Wet ice (international) |
Target Information
Src homology 2 (SH2) domains bind specifically to tyrosine-phosphorylated proteins that temporally participate in signal transduction events. Shc-like protein (Sck) is a neuronal adaptor protein containing an N-terminal PTB (phosphotyrosine binding) domain, a collagen homology (CH) domain, and a conserved C-terminal SH2 domain. Human Sck transcripts are highly expressed in liver, pancreas, prostate, and ovary. In vascular endothelial cells, Sck participates in VEGF-induced signal transduction. Treatment of human umbilical vein endothelial (HUVEC) cells with VEGF induces recruitment of Sck to tyrosine-1175 of the kinase insert domain-containing receptor (KDR) and enhances Sck tyrosine phosphorylation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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