Thermo Fisher Scientific Human IFN-gamma, Animal-Free Recombinant Protein, PeproTech
인간 IFN-γ 재조합 단백질로 면역세포 활성화 및 사이토카인 연구에 적합. E. coli 발현, ≥98% 순도, 낮은 엔도톡신(<0.1 EU/µg). HT-29 세포 기반 활성 검증, 동물 성분 무첨가. 연구용으로만 사용 가능.
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Applications
Functional Assay (Functional)
- Assay-dependent
In vitro Assay (IV)
- Refer to publications (72 available)
Miscellaneous PubMed (Misc)
- Refer to publications (5 available)
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Published Species | Bacteria, Fish, Human, Mouse, Rat, Virus |
| Expression System | E. coli |
| Amino Acid Sequence | MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ |
| Molecular Weight | 16.8 kDa |
| Class | Recombinant |
| Type | Protein |
| Purity | ≥ 98% by SDS-PAGE gel and HPLC analyses |
| Endotoxin Concentration | < 0.1 EU/µg |
| Activity | ED50 ≤ 0.05 ng/ml (specific activity ≥ 2 × 10⁷ units/mg, HT-29 cytotoxicity assay) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Purification | Purified |
| Contains | No preservative |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient |
Product Specific Information
Recombinant Human IFN-gamma is a 16.8 kDa protein containing 144 amino acid residues.
This product is shipped at ambient temperature. For storage, handling, and reconstitution information, please refer to the lot-specific Certificate of Analysis.
Target Information
IFN-gamma (Interferon gamma, Type II interferon) is a macrophage activation factor and immune interferon produced primarily by T-lymphocytes and natural killer cells in response to antigens, mitogens, and various cytokines.
- Dimeric protein consisting of two 146 amino acid subunits (~45 kDa functional homodimer).
- Appears as 25, 20, and minor 15.5 kDa bands on SDS-PAGE due to differential glycosylation.
- Highly species-specific; human IFN-gamma does not cross-react with mouse.
- Functions include antiviral activity, tumor antiproliferative effects, MHC induction, macrophage activation, and enhanced immunoglobulin secretion.
- Acts via IFN-gamma receptor I and II, activating the JAK-STAT pathway.
- Shares ~40% sequence homology with mouse IFN-gamma.
- Upregulated by IL-2, FGF basic, EGF; downregulated by vitamin D3 or DMN.
- Gene mutations associated with aplastic anemia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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