Thermo Fisher Scientific CD4 Monoclonal Antibody (RM4-4), PE-eFluor 610, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.25 µg/test
• Publications: View 3 publications

Product Specifications

Available Formats

• APC
• Biotin
• FITC
• PE
• Request custom conjugation

Product Specific Information

The RM4-4 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by most thymocytes, a subpopulation of mature T cells, and dendritic cells. CD4 binds to MHC class II on antigen-presenting cells and plays an important role in T cell development and function. In T cells, CD4 associates with the protein tyrosine kinase lck through its cytoplasmic tail. Binding of RM4-4 does not block binding of CD4 antibodies RM4-5 or GK1.5.

Applications Reported: Flow cytometric analysis
Applications Tested: Tested on mouse splenocytes; ≤0.25 µg/test (100 µL final volume, 10⁵–10⁸ cells/test). Optimize titration for best results.

Fluorochrome Information:
PE-eFluor 610 can be excited with 488–561 nm lasers and emits at 607 nm. Use a 610/20 band pass filter (equivalent to PE-Texas Red). Ensure compatibility with your instrument.

Light Sensitivity:
This tandem dye is sensitive to photo-induced oxidation. Protect vial and stained samples from light.

Fixation:
Samples can be stored in:

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-57)

Store up to 3 days at 4°C in the dark with minimal impact on brightness and FRET efficiency. Clone-specific performance should be verified empirically.

Excitation: 488–561 nm
Emission: 607 nm
Compatible Lasers: Blue, Green, Yellow-Green

Target Information

CD4 is involved in recognition of MHC class II molecules and functions as a co-receptor for HIV. It is primarily expressed on T helper cells but also found on monocytes, macrophages, and dendritic cells. CD4 expression is observed in thymus, lymph nodes, tonsils, spleen, and certain non-lymphoid tissues such as brain and gut. It aids T-cell activation via association with the T-cell receptor complex and Lck kinase. Multiple alternatively spliced transcripts are known (RefSeq, July 2017).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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