Thermo Fisher Scientific ATG7 Recombinant Rabbit Monoclonal Antibody (SC06-30)
ATG7 단백질을 인식하는 재조합 토끼 단일클론 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 높은 특이성과 민감도, 로트 간 일관성 및 동물 유래 성분이 없는 포뮬레이션을 제공. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:1,000–1:2,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 | - |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | View 1 publication |
| Flow Cytometry (Flow) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Published Species | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | SC06-30 |
| Immunogen | Synthetic peptide within Human Apg7 aa 654–703 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% Glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C, avoiding freeze/thaw cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2809507 |
Product Specific Information
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems developed by cloning specific antibody DNA sequences from immunoreactive rabbits. Individual clones are screened to select optimal candidates for production.
Advantages include:
- Improved specificity and sensitivity
- Lot-to-lot consistency
- Animal origin-free formulations
- Broader immunoreactivity to diverse targets due to larger rabbit immune repertoire
Target Information
The ATG7 gene was identified based on homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae APG7. In yeast, the protein is required for fusion of peroxisomal and vacuolar membranes. It shows homology to ATP-binding and catalytic sites of E1 ubiquitin activating enzymes.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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