Thermo Fisher Scientific FITC Monoclonal Antibody (1F8-1E4)

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

View 1 publication 1 publication

ELISA (ELISA)

1:50-1:200

-

Immunoprecipitation (IP)

1:50-1:200

-

Product Specifications

Species Reactivity

Chemical

Published species

Chemical

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

1F8-1E4

Conjugate

Unconjugated Unconjugated Unconjugated

View additional formats

• TRITC
• Request custom conjugation

Form

Liquid

Concentration

1.2 mg/mL

Purification

Chromatography

Storage buffer

PBS, pH 7.6

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_429692

Product Specific Information

Concentration may vary slightly from lot-to-lot, see lot-specific datasheet for exact concentration.

Product #31242 is recommended for use in ELISA, immunocytochemical, immunofluorescent, immunohistochemical and immunoprecipitation applications.

Storage: After opening, dilute only enough antibody for a single day`s use.

Country of Origin: USA

Target Information

FITC (fluorescein isothiocyanate) is a fluorochrome dye that absorbs ultraviolet or blue light causing molecules to become excited and emit a visible yellow-green light. This emission ceases upon removal of the light causing the excitation. Fluorochrome labeling provides rapid, accurate localization of antigen-antibody interaction when one of the reactants is part of a cell, tissue or other biological structure. FITC is a commonly used marker for antibodies in immunofluorescent techniques since the conjugation of FITC to proteins is relatively easy and does not, in general, destroy the biological activity of the labeled protein. FITC is widely used as a hapten to label different proteins. Anti-FITC antibodies can be used for isolating FITC labeled cells by magnetic microbeads based sorting, as well as removal of background signal.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.