Thermo Fisher Scientific H2BK12ac Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

ChIP assay (ChIP)

5 µg antibody for 5 µg-10 µg of Chromatin

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

A synthetic acetylated peptide around K12 of human Histone H2B (NP_003519.1). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:H2BK12ac,uniProtId:P62807-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_P62807-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_P62807-1_Rabbit_PDP.jpeg,sortOrder:1},{targetFamily:H2BK12ac,uniProtId:Q93079-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q93079-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q93079-1_Rabbit_PDP.jpeg,sortOrder:10},{targetFamily:H2BK12ac,uniProtId:Q99877-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q99877-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q99877-1_Rabbit_PDP.jpeg,sortOrder:11},{targetFamily:H2BK12ac,uniProtId:Q99879-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q99879-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q99879-1_Rabbit_PDP.jpeg,sortOrder:12},{targetFamily:H2BK12ac,uniProtId:Q99880-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q99880-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q99880-1_Rabbit_PDP.jpeg,sortOrder:13},{targetFamily:H2BK12ac,uniProtId:O60814-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_O60814-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_O60814-1_Rabbit_PDP.jpeg,sortOrder:2},{targetFamily:H2BK12ac,uniProtId:P06899-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_P06899-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_P06899-1_Rabbit_PDP.jpeg,sortOrder:3},{targetFamily:H2BK12ac,uniProtId:P23527-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_P23527-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_P23527-1_Rabbit_PDP.jpeg,sortOrder:4},{targetFamily:H2BK12ac,uniProtId:P33778-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_P33778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_P33778-1_Rabbit_PDP.jpeg,sortOrder:5},{targetFamily:H2BK12ac,uniProtId:P57053-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_P57053-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_P57053-1_Rabbit_PDP.jpeg,sortOrder:6},{targetFamily:H2BK12ac,uniProtId:Q16778-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q16778-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q16778-1_Rabbit_PDP.jpeg,sortOrder:7},{targetFamily:H2BK12ac,uniProtId:Q5QNW6-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q5QNW6-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q5QNW6-1_Rabbit_PDP.jpeg,sortOrder:8},{targetFamily:H2BK12ac,uniProtId:Q8N257-1,ncbiNodeId:9606,antigenRange:12,antigenLength:126,antigenImageFileName:PA5-109814_H2BK12ac_Q8N257-1_Rabbit.svg,antigenImageFileNamePDP:PA5-109814_H2BK12ac_Q8N257-1_Rabbit_PDP.jpeg,sortOrder:9}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.51 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2855225

Product Specific Information

Immunogen sequence: APKKG

Target Information

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The nucleosomes wrap further and compact DNA into chromotin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones play a central role in transciption regulation, DNA repair, DNA replication, and chromosomal stability. H2B has a broad antibacterial activity.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.