Thermo Fisher Scientific GFRAL Polyclonal Antibody
Thermo Fisher Scientific의 GFRAL Polyclonal Antibody는 Mouse GFRAL 단백질을 표적으로 하는 Sheep 유래 IgG 항체입니다. Western blot 및 다양한 IHC 응용에 적합하며, 높은 특이성과 낮은 교차반응을 제공합니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 2 µg/mL | - |
| Immunohistochemistry (IHC) | - | View 4 publications |
| Immunohistochemistry (Paraffin) (IHC (P)) | 5–15 µg/mL | - |
| Immunohistochemistry (Frozen) (IHC (F)) | 5–15 µg/mL | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Published Species | Mouse |
| Host / Isotype | Sheep / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHO-derived recombinant mouse GFR alpha-like isoform A (Gln20–Gly349) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 0.2 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS with 5% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, avoid freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607220 |
Product Specific Information
- In direct ELISA, less than 1% cross-reactivity with recombinant mouse GFR alpha-3 and GFR alpha-4 is observed.
- Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099).
Upon interaction with its ligand GDF15, GFRAL interacts with RET and induces cellular signaling through activation of MAPK and AKT pathways. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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