Thermo Fisher Scientific Calreticulin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

Assay-dependent

View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent

-

Product Specifications

Species Reactivity

Dog, Hamster, Human, Mouse, Rabbit, Rat

Published species

Amphibian, Human, Mouse, Rabbit, Rat

Host/Isotype

Chicken / IgY

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: D(399) E K E E D E E E S P G Q A K D(414). if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Calreticulin,uniProtId:P14211-1,ncbiNodeId:10090,antigenRange:399-414,antigenLength:416,antigenImageFileName:PA1-903_Calreticulin_P14211-1_Chicken.svg,antigenImageFileNamePDP:PA1-903_Calreticulin_P14211-1_Chicken_PDP.jpeg,sortOrder:2}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2228457

Product Specific Information

PA1-903 detects calreticulin from human, rat, mouse, hamster, rabbit and canine tissues.

PA1-903 has been successfully used in Western blot, immunoprecipitation, immunocytochemistry and immunofluorescence procedures. By Western blot, this antibody detects an ~60 kDa protein representing calreticulin and an ~120 kDa unidentified protein from rat brain extract.

PA1-903 immunizing peptide corresponds to amino acid residues 399-414 from mouse calreticulin. PA1-903 immunizing peptide (Cat. # PEP-017) is available for use in neutralization and control experiments.

Target Information

Calr or Calreticulin is a calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. Calr, a lectin, interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER. Calr also interacts with with the DNA-binding domain of NR3C1 and mediates its nuclear export. It may also be involved in maternal gene expression regulation and oocyte maturation via the regulation of calcium homeostasis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.