Thermo Fisher Scientific CD80 Monoclonal Antibody (17F3)

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1- 5 µg/mL

-

ELISA (ELISA)

0.01-0.05 µg/mL

-

Blocking Assay (BLOCK)

5-10 µg/mL

-

Product Specifications

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

17F3

Immunogen

Recombinant CD80 protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized antibody with deionized water (or equivalent) to a final concentration of 0.5 mg/ml.

CD80, also known as B7-1, is a single chain type I glycoprotein which belongs to the immunoglobulin superfamily. It is expressed on the surface of activated B and T cells, dendritic cells, and macrophages. It plays an important role in regulation of T cell activation.The interaction of CD80 with CD28 and CD152 (CTLA-4) induces and regulates immune responses.
Human CD80 Antibody (17F3), mAb, Mouse is produced from a hybridoma resulting from the fusion of partner and B-lymphocytes obtained from a mouse immunized with CD80.

Target Information

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28, and of an inhibitory receptor CTLA-4 (CD152). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, and both molecules can also substitute for each other in this process. CD80 is rapidly induced on the surface of in vitro activated B cells, with Epstein Barr Virus (EBV) transformed B cell lines, with Burkitts lymphoma cell lines, with freshly isolated follicular B lymphoma cells, T cells, and monocytes. The B-lymphocyte activation antigen B7-1 provides regulatory signals for T lymphocytes as a consequence of binding to the CD28 and CTLA4 ligands of T cells. Diseases associated with CD80 dysfunction include gallbladder squamous cell carcinoma and myocarditis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.