Thermo Fisher Scientific Zyxin Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:50-1:200

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 350-572 of human Zyxinin (NP_0034521) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Zyxin,uniProtId:Q15942-1,ncbiNodeId:9606,antigenRange:350-572,antigenLength:572,antigenImageFileName:PA5-120791_Zyxin_Q15942-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120791_Zyxin_Q15942-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.25 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2914363

Product Specific Information

Positive test controls include: HeLa. The target is usually found in the following locations: Cell junction, Cytoplasm, Nucleus, cytoskeleton, focal adhesion.

Immunogen sequence: PLTLKEVEEL EQLTQQLMQD MEHPQRQNVA VNELCGRCHQ PLARAQPAVR ALGQLFHIAC FTCHQCAQQL QGQQFYSLEG APYCEGCYTD TLEKCNTCGE PITDRMLRAT GKAYHPHCFT CVVCARPLEG TSFIVDQANR PHCVPDYHKQ YAPRCSVCSE PIMPEPGRDE TVRVVALDKN FHMKCYKCED CGKPLSIEAD DNGCFPLDGH VLCRKCHTAR AQT

Target Information

Zyxin is involved in focal adhesions, which are actin-rich structures that enable cells to adhere to the extracellular matrix and at which protein complexes involved in signal transduction assemble. Zyxin is a zinc-binding phosphoprotein that concentrates at focal adhesions and along the actin cytoskeleton. Zyxin has an N-terminal proline-rich domain and three LIM domains in its C-terminal half. The proline-rich domain may interact with SH3 domains of proteins involved in signal transduction pathways while the LIM domains are likely involved in protein-protein binding. Zyxin may function as a messenger in the signal transduction pathway that mediates adhesion-stimulated changes in gene expression and may modulate the cytoskeletal organization of actin bundles. Alternative splicing results in multiple transcript variants that encode the same isoform.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.