Thermo Fisher Scientific CD1d Monoclonal Antibody (1B1), Functional Grade, eBioscience

Applications and Tested Dilutions

Product Specifications

Available Formats

• Unconjugated
• Alexa Fluor 488
• APC
• Biotin
• Brilliant Violet 480
• Brilliant Violet 711
• eFluor 450
• NovaFluor Blue 660-120S
• NovaFluor Red 685
• NovaFluor Red 700
• NovaFluor Red 755
• NovaFluor Yellow 610
• NovaFluor Yellow 660
• NovaFluor Yellow 700
• NovaFluor Yellow 755
• PE
• PerCP-eFluor 710
• Super Bright 436
• Super Bright 600
• Super Bright 645
• Request custom conjugation

Product Specific Information

Description:
The 1B1 monoclonal antibody reacts with mouse CD1d, a 48 kDa glycoprotein structurally homologous to MHC class I molecules. CD1d associates with β2-microglobulin (β2m) and functionally resembles MHC class II. The 1B1 clone detects CD1d at varying levels on mouse leukocytes and recognizes β2m-associated CD1d.

Applications Reported:

• Flow cytometric analysis
• In vivo and in vitro studies

Applications Tested:
Tested by flow cytometric analysis of mouse splenocytes. Recommended use ≤1 µg/test (100 µL final volume). Optimal titration is advised per assay.

Storage and Handling:
Use in a sterile environment.

Filtration:
0.2 µm post-manufacturing filtered.

Purity:

90%, determined by SDS-PAGE.

Endotoxin Level:
<0.001 ng/µg antibody, determined by LAL assay.

Aggregation:
<10%, determined by HPLC.

Target Information

CD1d is a heavy chain associated with β2-microglobulin on cortical thymocytes. It can also be expressed independently on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family, which encodes transmembrane glycoproteins structurally related to MHC proteins. These proteins present lipid and glycolipid antigens to T cells. The CD1 family genes are located on chromosome 1 and vary in cellular localization and lipid ligand specificity. CD1d localizes to late endosomes and lysosomes via a tyrosine-based motif in its cytoplasmic tail. Diseases associated with CD1D include Mycobacterium malmoense infection and autoimmune disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.