Thermo Fisher Scientific Human ACVR1 [R206H], GST Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

147-end

Tag

GST-tag

Molecular weight

67.6 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM tris HCl, pH 7.5, with 10mM glutathione, 25% glycerol, 150mM NaCl, 0.1mM PMSF, 0.1mM EDTA, 0.25mM DTT

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Recombinant human ACVR1 (R206H) (147-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

• Validated in LanthaScreen® Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen® Eu Kinase Binding Assay

Protein Form: Recombinant

Assay Entry: Biochemical binding/kinetic

Target Information

ACVR1 (ALK2) belongs to the transforming growth factor-beta (TGF-beta) superfamily of structurally related signaling proteins which encodes activin A type I receptor. ACVR1 signals a particular transcriptional response in concert with activin type II receptors. Mutations in ACVR1 are associated with fibrodysplasia ossificans progressive (FOP) and the mutations are located in or adjacent to the GS regulatory region or active site of the kinase. The mutations in ACVR1 were predicted by protein structure homology modeling to activate the ACVR1 protein and enhance receptor signaling. Dysregulated ACVR1 kinase activity plays a role in the pathogenesis of FOP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.