Thermo Fisher Scientific MDFI Polyclonal Antibody
Thermo Fisher Scientific의 MDFI Polyclonal Antibody는 인간 MDFI 단백질을 인식하는 토끼 다클론 항체로, IHC(P) 및 ICC/IF 분석에 적합합니다. 항원 친화 크로마토그래피로 정제되었으며, PBS 버퍼(40% 글리세롤) 내 액상 형태로 제공됩니다. 연구용 전용 제품입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human MDFI. Recombinant protein control fragment (Product #RP-101208) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_2643822 |
Product Specific Information
Immunogen sequence:
LPNDSGHPSE LGGTRRAGNG ALGGPKAHRK LQTHPSLASQ GSKKSKSSSK STTSQIPLQA QEDCC
Highest antigen sequence identity to the following orthologs:
- Mouse: 67%
- Rat: 72%
Target Information
MDFI is a transcription factor that negatively regulates other myogenic family proteins. Studies of the mouse homolog, I-mf, show that it interferes with myogenic factor function by masking nuclear localization signals and preventing DNA binding. Knockout mouse studies show defects in the formation of vertebrae and ribs that also involve cartilage formation in these structures. MDFI can also interfere with the DNA-binding activity of Myod family members and plays an important role in trophoblast and chondrogenic differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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