Thermo Fisher Scientific CD10 Polyclonal Antibody, FITC
FITC 결합 CD10 폴리클로날 항체로 인간, 마우스, 랫트 반응성. 다양한 면역 분석(WB, IHC, ICC/IF, ELISA 등)에 사용 가능. 고순도 친화 크로마토그래피 정제. -20°C 암소 보관, 연구용 전용.
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Thermo Fisher Scientific CD10 Polyclonal Antibody, FITC
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:1,000 |
| Immunohistochemistry (IHC) | 1:50–1:250 |
| Immunocytochemistry (ICC/IF) | 1:50–1:250 |
| ELISA | 1:10,000 |
| Immunoprecipitation (IP) | 1:50–1:250 |
| Immunomicroscopy (IM) | 1:50–1:200 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host/Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide within amino acid region 100–150 on human Neprilysin (CD10) protein |
| Conjugate | FITC |
| Excitation/Emission Max | 498 / 517 nm |
| Form | Liquid |
| Concentration | 0.5–1.5 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 7.4–7.8, with 30% glycerol, 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
CD10 (Common Acute Lymphocytic Leukemia Antigen, CALLA) is a cell surface enzyme with neutral metalloendopeptidase activity that inactivates various biologically active peptides. It is expressed on lymphoblastic, Burkitt’s, and follicular germinal center lymphoma cells, immature B cells in adult bone marrow, and cells from patients with chronic myelocytic leukemia (CML).
CD10 is also present on breast myoepithelial cells, bile canaliculi, fibroblasts, and highly expressed on the brush border of kidney and gut epithelial cells.
This 100 kDa type II transmembrane glycoprotein cleaves peptides at the amino side of hydrophobic residues and inactivates peptide hormones such as glucagon, enkephalins, substance P, neurotensin, oxytocin, and bradykinin.
The CD10 gene has a 5′ untranslated region that is alternatively spliced into four mRNA transcripts; the coding region remains unaffected.
Diseases associated with CD10 dysfunction include spinocerebellar ataxia 43 and Charcot-Marie-Tooth disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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