Thermo Fisher Scientific ARAF Monoclonal Antibody (OTI2G9), TrueMAB
ARAF 단백질을 인식하는 Mouse monoclonal antibody(OTI2G9)로 Western blot 및 IHC(Paraffin)에 적합. Human ARAF(aa 145-365) 재조합 단백질로부터 유래. 액상형, 1 mg/mL 농도, PBS/BSA/glycerol buffer에 보관. -20°C에서 보관하며 동결-해동은 피해야 함.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:150 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI2G9 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 145–365 of human ARAF produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk), and Raf-1. Raf-1 is a cytoplasmic protein with intrinsic serine/threonine activity and is broadly expressed in nearly all cell lines tested to date. It is the cellular homolog of v-Raf, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-Raf protein has been associated with transformation and mitogenesis, while the activity of Raf-1 is normally suppressed by a regulatory N-terminal domain.
A-Raf, a second member of the Raf gene family of serine/threonine protein kinases, exhibits substantial homology to Raf-1 within the kinase domain but less homology elsewhere. Expression of A-Raf is found at highest levels in urogenital tissues and kidney and at lowest levels in brain tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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