Thermo Fisher Scientific Phospho-Bim (Ser118) Polyclonal Antibody
Bim 단백질의 Ser118 인산화 형태를 특이적으로 인식하는 폴리클로날 항체. 인간, 생쥐, 랫트 시료에 반응하며 WB 및 IHC(P) 실험에 적합. 고순도 정제 및 1 mg/mL 농도로 제공되며, -20°C에서 안정적으로 보관 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunohistochemistry (Paraffin) [IHC (P)]
- Tested Dilution: 1:50–1:200
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human BCL2L11 (Accession O43521), corresponding to amino acid residues around phosphorylated Ser118. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2816823 |
Product Specific Information
Antibody detects endogenous levels of Bim only when phosphorylated at Ser118.
Target Information
BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators involved in a wide variety of cellular activities. The protein encoded by this gene contains a Bcl-2 homology domain 3 (BH3). It interacts with other BCL-2 family members, including BCL2, BCL2L1/BCL-X(L), and MCL1, acting as an apoptotic activator. Expression can be induced by nerve growth factor (NGF) and the forkhead transcription factor FKHR-L1, suggesting a role in neuronal and lymphocyte apoptosis. Mouse transgenic studies indicate this gene functions as an essential initiator of apoptosis in thymocyte-negative selection. Several alternatively spliced transcript variants have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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