Thermo Fisher Scientific TrxR2 Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Antibody detects endogenous levels of total TXNRD2.

Target Information

The mammalian thioredoxin reductases (TrxRs) are a family of selenocysteine-containing pyridine nucleotide-disulfide oxidoreductases. All mammalian TrxRs are homologous to glutathione reductase in primary structure, including the conserved redox catalytic site (-Cys-Val-Asn-Val-Gly-Cys-), but distinctively possess a C-terminal extension containing a catalytically active penultimate selenocysteine (SeCys) residue in the conserved sequence (-Gly-Cys-SeCys-Gly-).

TrxR is a homodimeric protein; each monomer includes an FAD prosthetic group, a NADPH binding site, and a redox catalytic site. Electrons are transferred from NADPH via FAD and the active-site disulfide to the C-terminal SeCys-containing redox center, which then reduces substrates such as thioredoxin. Members of the TrxR family are 55–58 kDa in size and include three isoforms: cytosolic TrxR1, mitochondrial TrxR2, and TrxR3 (also known as Trx and GSSG reductase, TGR). TrxR plays a key role in cellular protection against oxidative stress and in redox regulation of transcription factors and various biological processes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.