Thermo Fisher Scientific BCKDHA Polyclonal Antibody
인체 BCKDHA 단백질을 인식하는 Rabbit Polyclonal 항체로, IHC(P)에서 1:200~1:500 희석 비율로 사용 가능. 항원 친화 크로마토그래피로 정제되었으며, PBS/glycerol buffer에 보관. 연구용으로만 사용.
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- PA557747
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- pk
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Applications
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:200–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human BCKDHA. Recombinant protein control fragment (Product #RP-95641) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.4 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2638503 |
Product Specific Information
Immunogen sequence:
EVNYWDKQDH PISRLRHYLL SQGWWDEEQE KAWRKQSRRK VMEAFEQAER KPKPNPNLLF SDVYQEMPAQ LRKQQESLAR HLQTYGEHYP LDH
- Highest antigen sequence identity to orthologs:
- Mouse: 94%
- Rat: 94%
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid dehydrogenase complex (BCKD; EC 1.2.4.4), an inner-mitochondrial enzyme complex that catalyzes the oxidative decarboxylation of branched-chain alpha-ketoacids derived from isoleucine, leucine, and valine.
This reaction is the second major step in the catabolism of branched-chain amino acids.
The BCKD complex consists of three catalytic components:
- Heterotetrameric (α2β2) branched-chain α-keto acid decarboxylase (E1)
- Homo-24-meric dihydrolipoyl transacylase (E2)
- Homodimeric dihydrolipoamide dehydrogenase (E3)
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme. The reaction is irreversible and constitutes the first committed step in BCAA oxidation. The BCKDHB gene encodes the beta subunit of E1. The complex also contains two regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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