Thermo Fisher Scientific SATB1 Polyclonal Antibody
Human, Mouse, Rat에 반응하는 Rabbit Polyclonal SATB1 항체로, WB, IHC, ICC/IF, ChIP 등 다양한 응용에 적합. 1 mg/mL 농도의 액상 제품이며, 항원 친화 크로마토그래피로 정제됨. 연구용으로만 사용 가능.
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Thermo Fisher Scientific SATB1 Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (IHC) | 2.5 µg/mL |
| Immunocytochemistry (ICC/IF) | 20 µg/mL |
| ChIP assay (ChIP) | 2.5 µg/10^6 cells |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | An 18 amino acid peptide near the amino terminus of the human SATB1 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS |
| Contains | 0.02% sodium azide |
| Storage Conditions | Maintain refrigerated at 2–8°C for up to 3 months; for long-term storage store at -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11152459 |
Product Specific Information
- Suggested positive control: SK-N-SH cell lysate
- PA5-20535 can be used with blocking peptide PEP-0655
Target Information
SATB1 (DNA-binding protein SATB1) is a crucial silencing factor involved in X inactivation mediated by Xist RNA during embryogenesis and lymphoma. It binds to AT-rich DNA sequences and nuclear matrix/scaffold-associated regions, recognizing the sugar-phosphate structure of double-stranded DNA. SATB1 acts as a transcriptional repressor, controlling nuclear and viral gene expression in a phosphorylation- and acetylation-dependent manner. It modulates genes essential for T-cell maturation and influences chromatin context targeted by HIV-1 integration machinery. HIV-1 Tat may overcome SATB1-mediated repression by binding to the same domain as HDAC1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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