Thermo Fisher Scientific Nestin Polyclonal Antibody
Nestin 단백질을 인식하는 Rabbit Polyclonal 항체로, 인간·생쥐·랫트 시료에 반응합니다. IHC, ICC/IF, Flow Cytometry, ELISA 등 다양한 응용에 사용 가능하며, 신경줄기세포 및 종양혈관 마커 연구에 적합합니다. 고순도 Protein A 정제, 1 mg/mL 농도, -20°C 보관.
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Thermo Fisher Scientific Nestin Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:300 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:100 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from rat Nestin (amino acids 681–750) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Nestin is a Class VI intermediate filament protein abundantly expressed in the developing central nervous system (CNS) and early embryonic neuroepithelial stem cells. It serves as a predominant marker for stem/progenitor cells, glioma cells, and tumor endothelial cells. Nestin is also a superior angiogenic marker for evaluating neovascularity in tumors. Specific antibodies to human Nestin help characterize progenitor cells differentiating into distinct lineages, enhance the therapeutic potential of neural stem/progenitor cells for CNS disease treatment, and identify neuroepithelial tumor cells. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations and is considered a novel angiogenesis marker in proliferating endothelial cells of colorectal cancer tissue.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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