Thermo Fisher Scientific EEF1B2 Polyclonal Antibody
Rabbit polyclonal antibody targeting human EEF1B2 protein. Validated for WB, IHC-P, and ICC/IF applications. High specificity with recombinant protein immunogen. Supplied in liquid form, unconjugated, purified by antigen affinity chromatography. Suitab...
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.04–0.4 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| Property | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human EEF1B2 (Recombinant protein control fragment Product #RP-102642) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.05 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2640893 |
Product Specific Information
Immunogen sequence:
FGDLKSPAGL QVLNDYLADK SYIEGYVPSQ ADVAVFEAVS SPPPADLCHA LRWYNHIKSY EKEKASLPGV KKALGKYGPA DVEDTTGSGA TD
Highest antigen sequence identity to orthologs:
- Mouse: 93%
- Rat: 91%
Target Information
EF-1 (elongation factor-1) is a multi-protein complex responsible for the delivery of aminoacyl-tRNAs to the ribosome. The EF-1 complex comprises four subunits: EF-1α, EF-1β, EF-1δ, and EF-1γ. EF-1β is a 225 amino acid protein belonging to the EF-1β/EF-1δ family and contains a GST C-terminal domain. EF-1β and EF-1δ stimulate GDP/GTP exchange on EF-1α, and phosphorylation of EF-1β affects this exchange rate. Alternative splicing of the EF-1β gene produces three transcript variants differing in the 5′ UTR.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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