Thermo Fisher Scientific Apolipoprotein B Polyclonal Antibody

Applications

• Western Blot (WB): 1:500–1:1,000
• View 2 publications

Product Specifications

Product Specific Information

• Purity is >95% by SDS-PAGE.

Target Information

Apolipoprotein B (apo B) in human plasma is a major protein of low-density lipoproteins (LDL) with a molecular mass of approximately 260–500 kDa. It binds to specific receptors on cell membranes and is involved in the removal of LDL and very low-density lipoprotein (VLDL) cholesterol from circulation.
LDL serves as the carrier protein for cholesterol in the blood and mediates uptake and clearance of cholesterol via receptor binding on capillary walls.
In atherosclerotic lesions, oxidatively modified LDL is found and recognized by macrophages through scavenger receptors A and CD36. Oxidized LDL is associated with atherosclerosis, although its exact modifications are not fully defined.
Apolipoprotein B is synthesized mainly in the liver and is a major component of very low, intermediate, and low-density lipoproteins, as well as lipoprotein (a). It serves as a ligand for the LDL receptor, and elevated levels are linked to premature atherosclerosis.
Normal plasma levels are around 800 mg/L. Two main isoforms exist: apoB-48 and apoB-100, both encoded by a single gene. ApoB-48 is produced by RNA editing of apoB-100 mRNA, introducing a stop codon at residue 2180.
Mutations in the Apolipoprotein B gene or its regulatory regions can cause disorders such as hypobetalipoproteinemia and hypercholesterolemia due to ligand-defective ApoB.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.