Thermo Fisher Scientific F(ab`)2-Goat anti-Human IgG Fc Secondary Antibody, Alexa Fluor 488

Applications

Tested Dilution

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Host/Isotype

Goat / IgG

Class

Polyclonal

Type

Secondary Antibody

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

View additional formats

• [Alexa Fluor 647 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor 647/host/Goat/)
• [Alexa Fluor Plus 488 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor Plus 488/host/Goat/)
• [Alexa Fluor Plus 647 ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Alexa Fluor Plus 647/host/Goat/)
• [AP](/antibody/secondary/product-name/Human IgG Fc/conjugates/AP/host/Goat/)
• [Biotin ](/antibody/secondary/product-name/Human IgG Fc/conjugates/Biotin/host/Goat/)
• [FITC](/antibody/secondary/product-name/Human IgG Fc/conjugates/FITC/host/Goat/)
• [HRP](/antibody/secondary/product-name/Human IgG Fc/conjugates/HRP/host/Goat/)
• [PE ](/antibody/secondary/product-name/Human IgG Fc/conjugates/PE/host/Goat/)
• [TRITC](/antibody/secondary/product-name/Human IgG Fc/conjugates/TRITC/host/Goat/)
• Request custom conjugation

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536548

Target

IgG Fc

Antibody Form

F(ab`)2 Fragment

Product Specific Information

Based on our testing, publications, and results reported from customers, the Alexa Fluor™ 488 dye provides the best fluorescein (FITC) substitute.

Target Information

Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

Analyte Specific Reagent