Thermo Fisher Scientific HYPE Monoclonal Antibody (3F5)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-5 µg/mL

-

ELISA (ELISA)

1 ng/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Antibody

Clone

3F5

Immunogen

HYPE (AAH01342, 1 a.a. approximately 449 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:FICD,uniProtId:Q9BVA6-1,ncbiNodeId:9606,antigenRange:1-458,antigenLength:458,antigenImageFileName:H00011153-M02_FICD_Q9BVA6-1_House_mouse.svg,antigenImageFileNamePDP:H00011153-M02_FICD_Q9BVA6-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Product Specific Information

Sequence of this protein is as follows: MAVTEPKWVS VWSRFLWVTL LSMVLGSLLA LLLPLGAVEE QCLAVLKGLY LLRSKPDRAQ HAATKCTSPS TELSITSRGA TLLVAKTKAS PAGKLEARAA LNQALEMKRQ GKREKAQKLF MHALKMDPDF VDALTEFGIF SEEDKDIIQA DYLYTRALTI SPYHEKALVN RDRTLPLVEE IDQRYFSIID SKVKKVMSIP KGNSALRRVM EETYYHHIYH TVAIEGNTLT LSEIRHILET RYAVPGKSLE EQNEVIGMHA AMKYINTTLV SRIGSVTISD VLEIHRRVLG YVDPVEAGRF RTTQVLVGHH IPPHPQDVEK QMQEFVQWLN SEEAMNLHPV EFAALAHYKL VYIHPFIDGN GRTSRLLMNL ILMQAGYPPI TIRKEQRSDY YHVLEAANEG DVRPFIRFIA KCTETTLDTL LFATTEYSVA LPEAQPNHSG FKETLPVKP

Target Information

Protein that can both mediate the addition of adenosine 5-monophosphate (AMP) to specific residues of target proteins (AMPylation), and the removal of the same modification from target proteins (de-AMPylation), depending on the context. The side chain of Glu-231 determines which of the two opposing activities (AMPylase or de-AMPylase) will take place. Acts as a key regulator of the ERN1/IRE1-mediated unfolded protein response (UPR) by mediating AMPylation or de-AMPylation of HSPA5/BiP (PubMed:25601083). In unstressed cells, acts as an adenylyltransferase by mediating AMPylation of HSPA5/BiP atThr-518, thereby inactivating it. In response to endoplasmic reticulum stress, acts as a phosphodiesterase by mediating removal of ATP (de-AMPylation) from HSPA5/BiP atThr-518`, leading to restore HSPA5/BiP activity. Although it is able to AMPylate RhoA, Rac and Cdc42 Rho GTPases in vitro, Rho GTPases do not constitute physiological substrates (PubMed:19362538, PubMed:25601083). [UniProt]

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.