Thermo Fisher Scientific CD103 Polyclonal Antibody
Rabbit polyclonal antibody against human CD103 (Integrin alpha E). Validated for IHC (paraffin). Supplied as liquid, 0.1 mg/mL in PBS with 40% glycerol. Suitable for research use in studying mucosal T lymphocytes and integrin-mediated adhesion.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| Category | Specification |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant protein corresponding to Human ITGAE (Integrin alpha E). Recombinant protein control fragment available as Product #RP-94973. |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | Store at 4°C for short term; for long term, store at -20°C. Avoid freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_2790476 |
Product Specific Information
Immunogen sequence:
CEEDCFSNAS VKVSYQLQTP EGQTDHPQPI LDRYTEPFAI FQLPYEKACK NKLFCVAELQ LATTVSQQEL VVGLTKELTL N
Target Information
CD103 (ITGAE, Integrin alpha E) is expressed on mucosa-associated T lymphocytes, activated cells, and subsets of TGF-β1 cells. It comprises a 150 kDa alpha chain and a 120 kDa beta chain, expressed by 0.5–2% of resting lymphocytes in peripheral blood and lymphoid organs. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes.
CD103 belongs to the integrin family—heterodimeric integral membrane proteins composed of alpha and beta chains. ITGAE encodes an I-domain-containing alpha integrin that undergoes post-translational cleavage, yielding disulfide-linked heavy and light chains. Together with beta 7 integrin, CD103 forms the E-cadherin-binding integrin known as the human mucosal lymphocyte-1 antigen. It is preferentially expressed in human intestinal intraepithelial lymphocytes (IEL) and may function in adhesion and IEL activation. CD103 dysfunction is associated with hairy cell leukemia and splenic marginal zone lymphoma.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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