Thermo Fisher Scientific Phospho-HTRA2 (Ser212) Polyclonal Antibody
HTRA2 단백질의 Ser212 인산화 형태를 특이적으로 검출하는 Rabbit Polyclonal Antibody. Western blot 및 IHC(P) 적용 가능. 고순도 정제 및 안정적 액상 형태로 제공되며, 연구용으로 세포 사멸 관련 경로 분석에 적합.
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Thermo Fisher Scientific Phospho-HTRA2 (Ser212) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000–1:3,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HTRA2 (Accession O43464), corresponding to amino acid residues around phosphorylated Ser212 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2903195 |
Product Specific Information
This antibody detects endogenous levels of HtrA2 only when phosphorylated at Ser212.
Target Information
The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif essential for their anti-apoptotic activity. A serine protease, Omi/HtrA2, is released from mitochondria upon induction of apoptosis and binds to IAP. The mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. Deregulated expression of Omi in the cytoplasm induces apoptosis, suggesting its role in the mitochondrial apoptotic pathway.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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