Thermo Fisher Scientific HGF Polyclonal Antibody
HGF 단백질을 인식하는 Rabbit Polyclonal Antibody로 Western blot, IHC, ICC/IF에 사용 가능. Human, Mouse, Rat 반응성. Affinity chromatography로 정제된 액상형 항체로, PBS와 50% glycerol에 보관. 세포 성장과 이동 조절 연구에 적합.
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Applications and Tested Dilution
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:2,000 | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
| Immunocytochemistry (ICC/IF) | 1:100–1:500 | - |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human HGF (Accession P14210), corresponding to amino acid residues D224–L274 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899990 |
Product Specific Information
Antibody detects endogenous levels of total HGF.
Target Information
Hepatocyte growth factor (HGF) regulates cell growth, motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor.
HGF is secreted by mesenchymal cells and acts as a multifunctional cytokine on epithelial cells. It stimulates mitogenesis, cell motility, and matrix invasion, playing a central role in angiogenesis, tumorigenesis, and tissue regeneration.
It is secreted as a single inactive polypeptide and cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the chains produces the active heterodimeric molecule.
The protein belongs to the plasminogen subfamily of S1 peptidases but lacks detectable protease activity. Alternative splicing produces multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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