Thermo Fisher Scientific T7-tag Polyclonal Antibody

Applications

Western Blot (WB)

• Tested Dilution: 1–3 µg/mL

Product Specifications

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to a final concentration of 0.5 mg/mL.

Well-characterized antibodies with useful epitope tags have been widely used in the study of protein expression in various systems.
The T7 tag (MASMTGGQQMG) is a peptide with 11 amino acids encoded in the leader sequence of T7 bacteriophage gene10, which encodes a T7 major capsid protein.
The T7 tag serves as a tag in many expression vectors, including the pET system that is based on the efficient T7 RNA polymerase expression system.
GenScript Rabbit Anti-T7-tag Polyclonal Antibody is developed in rabbit using a synthetic T7-tag epitope peptide conjugated to KLH.

Target Information

The T7 Tag is an 11 amino acid peptide derived from the bacteriophage T7 major capsid protein, gene 10.
T7 cleavage site: MASMTGGQQMG.

Epitope tags provide a method to:

• Localize gene products in various cell types
• Study protein topology and complexes
• Identify associated proteins
• Characterize newly identified or low-abundance proteins when specific antibodies are unavailable

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.