Thermo Fisher Scientific T7-tag Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

-

Product Specifications

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

T7-tag epitope peptide conjugated to KLH

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

Product Specific Information

Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.

Well-characterized antibodies with useful epitope tags have been widely used in the study of protein expression in various systems.The T7 tag (MASMTGGQQMG) is a peptide with 11 amino acids encoded in the leader sequence of T7 bacteriophage gene10. This gene encodes a T7 major capsid protein.The T7 tag serves as a tag in many expression vectors including the pET system that is based on the efficient T7 RNA polymerase expression system.GenScript Rabbit Anti-T7-tag Polyclonal Antibody is developed in rabbit using a synthetic T7-tag epitope peptide conjugated to KLH.

Target Information

The T7 Tag is an 11 amino acid peptide derived from the bacteriophage T7 major capsid protein, gene 10. T7 cleavage site: MASMTGGQQMG. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.