Thermo Fisher Scientific DUSP1/MKP1 Polyclonal Antibody
DUSP1/MKP1 단백질을 인식하는 Rabbit Polyclonal 항체로, 인간, 생쥐, 랫트에 반응합니다. Western blot, IHC, ICC/IF, ELISA 등 다양한 응용에 사용 가능하며, Protein A 정제 및 1 mg/mL 농도의 액상 형태로 제공됩니다. 연구용으로만 사용됩니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:200 |
| Immunohistochemistry (Frozen) (IHC-F) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH-conjugated synthetic peptide derived from human DUSP1 (amino acids 151–250) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | –20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The expression of the DUSP1 gene is induced in human skin fibroblasts by oxidative or heat stress and growth factors. It encodes a protein with structural features similar to non-receptor-type protein-tyrosine phosphatases and shows significant amino acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by dephosphorylating both phosphothreonine and phosphotyrosine residues. It also suppresses MAP kinase activation by oncogenic ras in Xenopus oocyte extracts. Therefore, DUSP1 may play an important role in the human cellular response to environmental stress and in the negative regulation of cellular proliferation.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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