Thermo Fisher Scientific YKL-40 (CHI3L1) Human ProcartaPlex Simplex Kit

Invitrogen™ YKL-40 (CHI3L1) Human ProcartaPlex™ Simplex Kit

The Human YKL-40 (CHI3L1) Simplex ProcartaPlex Kit measures YKL-40 (CHI3L1) protein and is designed to be combinable with other Simplex kits, allowing you to create a custom multiplex panel utilizing Luminex xMAP technology for protein detection and quantitation.
When combining multiple Simplex kits (i.e., not using a pre-configured Multiplex Panel), only one buffer kit (sold separately) is required per assay plate regardless of plex size.

About ProcartaPlex Assays for the Luminex Platform

ProcartaPlex immunoassays are based on the sandwich ELISA principle, using two highly specific antibodies binding to different epitopes of one protein to quantify multiple protein targets simultaneously using a Luminex instrument.
ProcartaPlex multiplex assays require as little as 25 μL of plasma or serum, or 50 μL of cell culture supernatant, and yield analyzed results within four hours.

• More results per sample — measure multiple protein targets simultaneously in a single 25–50 μL sample
• Well-established Luminex technology — highly referenced multiplexing platform for protein detection and quantitation

ProcartaPlex assays utilize Luminex xMAP (multianalyte profiling) technology for simultaneous detection and quantitation of up to 80 protein targets in a single 25–50 μL sample from plasma, serum, cell culture supernatants, and other biological fluids.

The Luminex beads in the ProcartaPlex assay are internally dyed with precise proportions of red and infrared fluorophores, creating spectrally unique signatures identifiable by Luminex xMAP detection systems (e.g., Luminex 200, FLEXMAP 3D, MAGPIX).
Similar to a sandwich ELISA, matched antibody pairs identify the protein of interest. Each spectrally unique bead is labeled with antibodies specific to a single target protein, and bound proteins are detected with biotinylated antibodies and streptavidin–R-phycoerythrin (RPE). This allows analysis of multiple targets in a single well.

The key difference between a ProcartaPlex assay and ELISA is that the capture antibody in ProcartaPlex is conjugated to a bead rather than adsorbed to a microplate well, allowing reagents to remain free-floating in solution.
Luminex instruments use dual lasers — one to distinguish bead signatures and another to quantify RPE fluorescence proportional to protein concentration.
ProcartaPlex multiplex assays can profile more target proteins using significantly less sample in the same time as a traditional sandwich ELISA.

ProcartaPlex multiplex panels are available in multiple formats across six species (human, mouse, rat, nonhuman primate, porcine, and canine).
For more information, visit thermofisher.com/procartaplex.

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