Thermo Fisher Scientific JAZF1 (Transcription Corepressor) Monoclonal Antibody (PCRP-JAZF1-1C2)
JAZF1 단백질을 인식하는 마우스 모노클로날 항체로, Western blot, IHC, ICC, Flow cytometry 등 다양한 응용에 적합. 핵 내 전사 억제 단백질 검출에 사용. HeLa 및 U87 세포 양성 대조로 권장. 단백질 A/G로 정제된 액상 형태.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1–2 µg/mL |
| Immunohistochemistry (Paraffin) (IHC-P) | 1–2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–2 µg/mL |
| Flow Cytometry (Flow) | 1–2 µg/10⁶ cells |
| Immunoprecipitation (IP) | 1–2 µg/100–500 µg lysate |
| Peptide Array (Array) | Assay-dependent |
| Immunoelectrophoresis (IE) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PCRP-JAZF1-1C2 |
| Immunogen | Recombinant fragment (aa 1–130) of human JAZF1 protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 200 µg/mL |
| Purification | Protein A/G |
| Storage Buffer | PBS with 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
- Antibody stability: 24 months
- Positive control: HeLa or U87 cells
- Expression: Ubiquitous tissue expression
- Cellular localization: Nucleus
- Specificity: JAZF1 (juxtaposed with another zinc finger protein 1), also known as TIP27 or ZNF802, is a 243 amino acid nuclear protein containing three C2H2-type zinc fingers.
- Function: Acts as a transcriptional repressor by interacting with nuclear orphan receptor TR4, downregulating its expression.
- Clinical relevance: Chromosomal aberrations in JAZF1 are associated with endometrial stromal tumors, suggesting a role in carcinogenesis.
- Predicted to react in: Mouse
Target Information
JAZF1 is a nuclear protein with three C2H2-type zinc fingers functioning as a transcriptional repressor. Chromosomal aberrations involving this gene are linked to endometrial stromal tumors. Multiple alternatively spliced variants exist, encoding different isoforms, though not all have been fully characterized.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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