Invitrogen™

Click-IT™ L-Homopropargylglycine (HPG)

Click-iT® homopropargylglycine (HPG) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional technique,35S methionine for the자세히 알아보기

Click-iT® homopropargylglycine (HPG) provides a fast, sensitive, non-toxic and most importantly non-radioactive alternative to the traditional technique,35S methionine for the detection of nascent protein synthesis. HPG is an amino acid analog that contains a very small modification, specifically an alkyne moiety that can be fed to cultured cells and incorporated into proteins during active protein synthesis. Detection utilizes the chemoselective ligation or “click` reaction between and azide and an alkyne where the alkyne modified protein is detected with an azide-containing dye or hapten together with either the Click-iT® Cell Reaction Buffer Kit or the Click-iT® Protein Buffer Kit. With the Click-iT® Cell Reaction Buffer Kit, cells can be analyzed by fluorescence microscopy, flow cytometry or high-content imaging and analysis. With the Click-iT® Protein Reaction Buffer Kit, achieve detection sensitivity in 1-D gels and western blots in the low femtomole range or perform LC-MS⁄MS and MALDI MS analysis.

사양

그린 기능Less hazardous

제품 유형L-Homopropargylglycine

수량5 mg

라벨링 방법Metabolic Labeling

배송 조건Room Temperature

제품라인Click-iT™, Molecular Probes™

라벨 또는 염료Alkyne

형식Solid

Labeling TargetProteins

Unit SizeEach