Thermo Fisher Scientific ATG12 Polyclonal Antibody
Rabbit polyclonal antibody against human ATG12 protein. Validated for ICC/IF applications. Recognizes recombinant ATG12 antigen (aa 97–139). Supplied as liquid, 0.2 mg/mL in PBS with 40% glycerol. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Immunocytochemistry (ICC/IF) | 0.25–2 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence (range: 97–139). Recombinant protein control fragment: Product #RP-109913 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 40% glycerol |
| Contains | 0.02% sodium azide |
| Storage conditions | Store at 4 °C short term. For long-term storage, store at −20 °C, avoiding freeze/thaw cycles. |
| Shipping conditions | Wet ice |
| RRID | AB_3091834 |
Product Specific Information
- Immunogen sequence: SEQLFIYVNQ SFAPSPDQEV GTLYECFGSD GKLVLHYCKS QAW
- Antigen sequence identity: Mouse 100%; Rat 100%
Target Information
ATG12, a member of the autophagy protein family, forms a conjugate with ATG5 that exhibits E3-like ubiquitin-protein ligase activity for protein lipidation in autophagy. This conjugate also interacts with innate immune response proteins such as RIG-I and VISA (IPS-1), inhibiting type I interferon production and allowing viral replication. ATG12 interacts with ATG10 in human embryonic kidney cells in the presence of ATG7. At least two isoforms of ATG12 are known. Autophagy, the lysosomal degradation pathway, is essential for growth control and nutrient preservation under starvation and may be defective in tumor cells. This process is negatively regulated by TOR through phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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