Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Yellow 660, eBioscience

Thermo Fisher Scientific CD16 Monoclonal Antibody (3G8), NovaFluor Yellow 660

Applications

• Flow Cytometry (Flow)

Tested Dilution: 5 µL (0.2 µg)/test

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Product Specifications

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Additional Formats

• APC-eFluor 780
• Functional Grade
• NovaFluor Blue 510 / 530 / 585 / 610-70S / 660-120S / 660-40S / 760
• NovaFluor Red 685 / 700 / 725 / 755
• NovaFluor Yellow 590 / 610 / 690 / 700 / 730 / 755
• PerCP-eFluor 710
• Super Bright 436 / 600 / 645 / 702
• Request custom conjugation available

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Product Specific Information

This 3G8 monoclonal antibody reacts with human and non-human primate CD16 (low-affinity Fc gamma RIII).
CD16 exists as two isoforms: FcγRIIIA and FcγRIIIB.

• FcγRIIIA: Expressed on monocytes, macrophages, and NK cells.
• FcγRIIIB: Detected on neutrophils as a GPI-anchored form.

Binding of IgG activates signal transduction pathways leading to:

• Antibody-dependent cell-mediated cytotoxicity (ADCC)
• Phagocytosis
• Cytokine release
• Antigen presentation

3G8 recognizes the same epitope as CB16 but distinct from B73.1.
Each product includes 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer.

Applications Reported: Flow cytometric analysis
Applications Tested: Pre-diluted and validated for flow cytometry of human peripheral blood cells (5 µL/test, 100 µL final volume, 10⁵–10⁸ cells/test).

NovaFluor Compatibility Notes:

• Not compatible with DNA intercalating viability dyes (propidium iodide, 7-AAD, DAPI).
• Recommended: Invitrogen LIVE/DEAD Fixable Dead Cell stains.

CellBlox Plus Blocking Buffer (Cat. No. C001T06F01):

• Required for optimal NovaFluor staining.
• Add 5 µL per sample before combining with cells.
• For single-color controls, use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor Technology:
Based on Phiton technology utilizing nucleic acid dye structures for engineered fluorescent signatures minimizing spillover and spread.

Excitation: 552 nm
Emission: 663 nm
Laser: 561 nm (Yellow)

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Target Information

CD16 encodes a receptor recognizing the Fc portion of IgG, involved in:

• Clearance of immune complexes
• Cellular cytotoxicity
• Enhancement of viral infection

Gene: FCGR3A (chromosome 1)
Isoforms:

• FCGR3A: NK cells, transmembrane anchored
• FCGR3B: Neutrophils, PI linkage

Mutations associated with:

• Immunodeficiency 20
• Herpes Zoster
• Systemic lupus erythematosus susceptibility
• Alloimmune neonatal neutropenia

Alternative splicing generates multiple isoforms.

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For Research Use Only.
Not for use in diagnostic procedures or resale without authorization.

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